HPLC and UPLC Columns

Puritas 120Å C-4

 

Overview

PURITASTM C-4 phases are bonded with butyl groups and show moderate hydrophobicity. They are suited for the separation of peptides and proteins. Especially,larger pore silicas with less surface area are used to avoid denaturation of proteins. The phases are also useful for separating components within a wide range of hydrophobicity in one shot.

Key Features

  • Recommended for biological separations
  • Quick separation of samples with wide range of hydrophobicity

 

 

 

 

 


Specifications

 

ANALYSIS SPECIFICATION
Average particle size *) 5.0μm
Average pore size 120Å
Specific surface area (m2/g) 300
Average pore volume (ml/g) 1.00
Carbon content (%) 7

*)The Specification of particle size and physical properties are those of base silicagel

 

 


APPLICATION: LOCAL ANAESTHETICS 4.6mm x 250mm, 120Å, 5µm

Chromachemie Proprietary information

 

 

 

CONDITIONS
Mobile phase:
A. 50mM NaH2PO4 (pH-2.5):
ACN (95:5)
B. 50mM NaH2PO4 (pH-2.5):
ACN (47:53)
Gradient: 0-100% B in 30 min,
100% B 30-45 min
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Xylocaine
2. Benzocaine
3. Cinchocaine
4. Butamben


Ordering Information

 

Product code Column Dimensions Particle Size (µm) Bonding Phase
PN04-05-120-250 250mm x 4.6mm 5 C4
PN04-05-120-150 150mm x 4.6mm 5 C4
PN04-05-120-100 100mm x 4.6mm 5 C4
PN04-05-120-050 50mm x 4.6mm 5 C4