HPLC and UPLC Columns

Puritas BIO eximius 300Å C18



The 21st century is often considered as the era of “protein science”. Peptide and protein research is quickly maturing to be the major driving force for new kinds of pharmaceutical ingredients. For both, the research and the big scale of peptide and protein manufacturing spherical silica must be tailor made, fine tuned. The new line up of PURITASTM BIO EXIMIUS is designed to serve these needs, featuring extended acidic and alkalic resistance and high loadability combined with exceptional durability.

The improved high density bonding and full end capping makes it very suitable to general use and to separate or purify high molecular weight compounds, especially proteins. Because of the significant improvement in durability and acidic, alkalic resistance it can be used for extended period of time in acidic mobile phase condition and rinsed for recovery with NaOH containing buffer.

Key Features

  • Recommended for highly hydrophobic bulky molecules
  • Novel chemical bonding assures stability under severe alkalic and acidic conditions
  • Designed for protein purification
  • 30nm pore size, narrow distribution range
  • Totally spherical particles made of ultra high purity silica gel
  • Featuring extended acidic and alkalic resistance and high durabilit



Average particle size *) 5.0μm
Average pore size 300Å
Specific surface area (m2/g) 100
Average pore volume (ml/g) 0.8
Particle size distribution (D40/D90) 8

*)The Specification of particle size and physical properties are those of base silicagel



APPLICATION: HUMAN INSULIN 4.6mm x 250mm, 300Å, 5µm

Chromachemie Proprietary information




Mobile phase:
A: 0.1% TFA in H2O:ACN (80:20)
B: 0.1% TFA in H2O:ACN (10:90)
Gradient: 0-60% B in 15 min
Flow Rate: 1ml/min
Injection Volume: 20µl
Detection: 280nm

1. Human Insulin

Ordering Information


Product code Column Dimensions Particle Size (µm) Bonding Phase
PBE18-05-300-250 250mm x 4.6mm 5 C18
PBE18-05-300-150 150mm x 4.6mm 5 C18
PBE18-05-300-100 100mm x 4.6mm 5 C18
PBE18-05-300-050 50mm x 4.6mm 5 C18
PBE18-10-300-250 250mm x 4.6mm 10 C18