HPLC and UPLC Columns

Puritas APS

 

Overview

PURITASTM APS-P phases are bonded with amino-propyl silane using our ultra pure spherical silica gel as base material. Depending on the choice of the eluent, it can both be used under normal andreversed phase mode. Especially, it is useful for saccharide separation using acetonitrile - water. The phase is also recommended for the separation of basic compounds under normal phase conditions, which could not be separated using unmodified silica gel. Simple eluents such as hexane - ethyl acetate or chloroform / methanol are used for the separation without any polar solvent additives.

Key Features

  • Most versatile medium polar phase
  • Useful in both normal and reversed phase
  • Useful as a support of special modification
  • Recommended for basic compounds separation

Specifications

 

ANALYSIS SPECIFICATION
Average particle size *) 5.0μm
Average pore size 120Å
Specific surface area (m2/g) 320
Average pore volume (ml/g) 1.06
Carbon content (%) 4.8

*)The Specification of particle size and physical properties are those of base silicagel

 

 


APPLICATION: SUBSTITUTED BENZENES 4.6mm x 250mm, 120Å, 5µm

Chromachemie Proprietary information

 

 

 

CONDITIONS
Mobile phase:
Hexane: CH2Cl2 (97:3)
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Butylbenzene
2. Methylbenzoate
3. Nitrobenzene

 

APPLICATION: SUBSTITUTED BENZENES 4.6mm x 250mm, 120Å, 5µ

Chromachemie Proprietary information

 

 

 

CONDITIONS
Mobile phase: ACN:20mM KH2PO4,
pH-7.8 (80:20)
Flow Rate: 1.0ml/min
Detection: UV, 270nm

COMPOUNDS
1. Caffeine
2. Thymine
3. Uracil


Ordering Information

 

Product code Column Dimensions Particle Size (µm) Bonding Phase
PNAP-05-120-250 250mm x 4.6mm 5 APS
PNAP-05-120-150 150mm x 4.6mm 5 APS
PNAP-05-120-100 100mm x 4.6mm 5 APS
PNAP-05-120-050 50mm x 4.6mm 5 APS