Puritas Eximius C-18

Overview

The Puritas Eximius represents a high performance ODS phase based on a new generation of silica gel developed to show long peak retention and high loadability, as a result of its exceptionally high surface area.

The ODS bonding density is chosen with respect to optimal selectivity for both hydrophilic and hydrophobic compounds, enabling even the use of 100% aqueous eluents. Our proprietary end capping technology minimizes residual silanol groups amount below the detectable level. This result in very good peak symmetry, particularly in case of basic compounds and exhibits high chemical phase robustness.

Puritas ODS series is available with particle sizes of 2.1, 3, 5, 10 and 15 microns for both analytical as well as preparative applications.

Key Features

  • Ultra high purity silica
  • High surface area and optimum porosity
  • Narrow pore size distribution
  • Superior Loadability and Surface Area Accessibility
  • Easy Scale Up and Availability in Large Quantity
  • Mechanical Strength of Base Silica
  • Higher pH stability
  • Superior performance for both hydrophilic and hydrophobic compounds
  • Minimal silanol activity due to our new proprietary end capping technology

Specifications

  • Analysis

  • Particle size (*)
  • Average D50 (µm)
  • Distribution D40/D90
  • Physical property
  • Pore size
  • Sp. Surface Area (m2/g)
  • Pore Volume (ml/g)
  • Elemental analysis
  • Carbon content (%)
  • Specification

  • 5.0µm
  • 4.2-4.8
  • ≤1.25
  •  
  • 100Å
  • 400-480
  • 1.00-1.20
  •  
  • 15.5-17.5
  • Method

  • Sedimentation method
  • Sedimentation method
  • Sedimentation method
  • B.E.T.
  • B.E.T.
  • B.E.T.
  • B.E.T.
  •  
  •  

*)The Specification of particle size and physical properties are those of base silicagel

Application: Aflatoxin ANALYSIS 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Column: 4.6mm X 150mm,
C18, 5µm
Mobile phase: H2O: MeOH:
ACN (60:30:15)
Flow Rate: 1.0ml/min
Detection: FLD, Ex=365, Em=464

COMPOUNDS
1. Aflatoxin B1
2. Aflatoxin B2
3. Aflatoxin G1
4. Aflatoxin G2

Application: maleic and fumaric acids 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 50mM KH2PO4 (pH 7.0)
Flow Rate: 1.0ml/min
Detection: UV,210nm

COMPOUNDS
1. Fumaric acid
2. Maleic acid

Application: Nitroanilines 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN:50mM KH2PO4, pH 7.0 (50:50)
Flow Rate: 1.0ml/min
Detection: UV,254nm

COMPOUNDS
1. p-Nitroaniline
2. m-Nitroaniline
3. o-Nitroaniline

Application: Organic Acids 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: MeOH:50mM KH2PO4 , pH 5.7 (30:70)
Flow Rate: 1.0ml/min
Detection: UV,220nm

COMPOUNDS
1. L-Ascorbic acid
2. Maleic acid
3. Acetyl salicylic acid
4. Benzoic acid
5. Salicylic acid

Application: Food Additives 4.6mm x 250mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN:50mM KH2PO4, pH 4.4 (40:60)
Flow Rate: 1.0ml/min
Detection: UV,230nm

COMPOUNDS
1. Phthalic acid
2. P-Hydroxy benzoic acid
3. Benzoic acid
4. Sorbic acid
5. Methyl paraben
6. Ethyl paraben
7. Propyl paraben

Application: Chlorphenamine maleate Impurities 4.6mm x 250mm, 100Å, 10µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN/NH4H2PO4, pH 3.0 (20:80)
Flow Rate: 1.2ml/min
Detection: UV,225nm

COMPOUNDS
1. Maleic acid
2. Chlorphenamine maleate imp. B
3. Chlorphenamine maleate imp. C
4. Chlorphenamine maleate API
5. Chlorphenamine maleate imp. D

Application: Ibuprofen impurities 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A: 0.5 ml H3PO4+340 ml ACN + 660 ml H2O
B: ACN
Gradient: 0-25 min 100% A,
25-55 min 0-85% B,
55-70 min 85% B
Flow Rate: 2 ml/min
Detection: UV, 214 nm

COMPOUNDS
1. Ibuprofen imp. A
2. Ibuprofen imp. B
3. Ibuprofen API

Application:
Dextromethorphan hydrobromide IMpurities 4.6mm x 250mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 3.11 g of docusate sodium in
400 ml H2O+ 600ml ACN + 0.56 g
NH4NO3 (pH-2.0)
Flow Rate: 1.0ml/min
Detection: UV,280nm

COMPOUNDS
1. Dextromethorphan hydrobromide imp. B
2. Dextromethorphan hydrobromide imp. C
3. Dextromethorphan hydrobromide API
4. Dextromethorphan hydrobromide imp. A

Application: paracetamol impurities 4.6mm x 250mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 375 ml of 17.9 g/L Na2HPO4
+375 ml of 7.8 g/L
NaH2PO4 + 250 ml MeOH containing 4.6 g/L of
Tetrabutylammonium hydroxide
Flow Rate: 1.0ml/min
Detection: UV,280nm

COMPOUNDS
1. Paracetamol imp. K
2. Paracetamol API
3. Paracetamol imp. F

Ordering Information

Product code Column Dimensions Particle Size (µm) Bonding Phase
PE18-05-100-250 250mm x 4.6mm 5 ODS (C18)
PE18-05-100-150 150mm x 4.6mm 5 ODS (C18)
PE18-05-100-100 100mm x 4.6mm 5 ODS (C18)
PE18-05-100-050 50mm x 4.6mm 5 ODS (C18)
PE18-03-100-250 250mm x 4.6mm 3 ODS (C18)
PE18-03-100-150 150mm x 4.6mm 3 ODS (C18)
PE18-03-100-100 100mm x 4.6 mm 3 ODS (C18)
PE18-03-100-050 50mm x 4.6mm 3 ODS (C18)
PE18-2.1-100-100 100mm X 4.6mm 2.1 ODS (C18)
PE18-2.1-100-050 50mm X 4.6mm 2.1 ODS (C18)
PE18-10-100-250 250mm x 4.6mm 10 ODS (C18)