HPLC Columns

PuritasTM Columns Offer

Excellent Mechanical Strength

  • Ultra high purity silica with completely spherical, totally porous particle
  • Well controlled narrow particle size distribution

Outstanding Chemical Stability

  • High bonding density
  • Absolutely minimal silanol activity due to new proprietary end capping technology

Superior Loadability and Surface Area Accessibility

  • Optimized particle surface area, pore volume, pore diameter and its distribution to give good surface morphology

Easy Scale Up and Availability in Bulk Quantity

  • The same selectivity, retention characteristics and high efficiency from Analytical to Preparative and Process scale
  • Well experienced and well controlled Large scale silica manufacturing in bulk batches

Excellent Homogeneity and Batch to Batch Reproducibility

  • Quality carefully monitored and very tightly controlled in each step of manufacturing by QbD
  • Manufactured from ISO 9001 certified bare silica and bonded phases originating from GMP compliant chemical bonding process

THE PURITY OF BASE SILICA

The most important feature of the silica used in column manufacturing is low metal impurities content. If metal impurities are present, there is possibility of peak deterioration (tailing, low peak height etc.) which results in lower sample recoveries. The purity of silica used in manufacturing of PURITASTM columns can be underlined by the lowest metal impurities content as shown below in the table

Al Ti Fe Zr
Acceptable Standard Values (ppm) <10.0 <10.0 <10.0 <10.0
Measured Values (ppm) <1.0 <1.0 <1.0 <1.0

NEW BONDING TECHNOLOGY

The New bonding technology minimizes free residual silanol interactions by removing almost all free silanol groups as shown in the following graph

PURITASTM-ODS: SILANOL DETERMINATION BY 29Si-MASNMR

BEFORE BONDING PURITASTM ODS

BEFORE BONDING PURITAS<sup>TM</sup> ODS

AFTER BONDING PURITASTM ODS

AFTER BONDING PURITAS<sup>TM</sup> ODS

HIGH SURFACE AREA (EXTRAORDINARY LOADABILITY)

PURITASTM ODS EXTRA ORDINARY LOADABILITY

Grade Maximum
loadability (mg)
Puritas ODS 19.7
Kromasil-100-C18 10.6
Inertsil-ODS-3V 8.2
Symmetry-C18 5.8

RESOLUTION-LOADABILITY CORRELATION

AFTER BONDING PURITAS<sup>TM</sup> ODS

HIGH STABILITY OVER WIDE PH RANGE

ACIDIC RESISTANCE (pH=1)

AFTER BONDING PURITAS<sup>TM</sup> ODS

ALKALIC RESISTANCE (pH=12)

AFTER BONDING PURITAS<sup>TM</sup> ODS