PuritasTM Eximius 100Å C-18

Application: Aflatoxin ANALYSIS 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Column: 4.6mm X 150mm,
C18, 5µm
Mobile phase: H2O: MeOH:
ACN (60:30:15)
Flow Rate: 1.0ml/min
Detection: FLD, Ex=365, Em=464

COMPOUNDS
1. Aflatoxin B1
2. Aflatoxin B2
3. Aflatoxin G1
4. Aflatoxin G2

Application: maleic and fumaric acids 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 50mm KH2PO4 (pH 7.0)
Flow Rate: 1.0ml/min
Detection: UV,210nm

COMPOUNDS
1. Fumaric acid
2. Maleic acid

Application: Nitroanilines 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN:50mm KH2PO4, pH 7.0 (50:50)
Flow Rate: 1.0ml/min
Detection: UV,254nm

COMPOUNDS
1. p-Nitroaniline
2. m-Nitroaniline
3. o-Nitroaniline

Application: Organic Acids 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: MeOH:50mm KH2PO4 , pH 5.7 (30:70)
Flow Rate: 1.0ml/min
Detection: UV,220nm

COMPOUNDS
1. L-Ascorbic acid
2. Maleic acid
3. Acetyl salicylic acid
4. Benzoic acid
5. Salicylic acid

Application: Food Additives 4.6mm x 250mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN:50mm KH2PO4, pH 4.4 (40:60)
Flow Rate: 1.0ml/min
Detection: UV,230nm

COMPOUNDS
1. Phthalic acid
2. P-Hydroxy benzoic acid
3. Benzoic acid
4. Sorbic acid
5. Methyl paraben
6. Ethyl paraben
7. Propyl paraben

Application: Chlorphenamine maleate Impurities 4.6mm x 150mm, 100Å, 10µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN/NH4H2PO4, pH 3.0 (20:80)
Flow Rate: 1.2ml/min
Detection: UV,225nm

COMPOUNDS
1. Maleic acid
2. Chlorphenamine maleate imp. B
3. Chlorphenamine maleate imp. C
4. Chlorphenamine maleate API
5. Chlorphenamine maleate imp. D

Application: Ibuprofen impurities 4.6mm x 150mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A: 0.5 ml H3PO4+340 ml ACN + 660 ml H2O
B: ACN
Gradient: 0-25 min 100% A,
25-55 min 0-85% B,
55-70 min 85% B
Flow Rate: 2 ml/min
Detection: UV, 214 nm

COMPOUNDS
1. Ibuprofen imp. A
2. Ibuprofen imp. B
3. Ibuprofen API

Application:
Dextromethorphan hydrobromide IMpurities 4.6mm x 250mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 3.11 g of docusate sodium in
400 ml H2O+ 600ml ACN + 0.56 g
NH4NO3 (pH-2.0)
Flow Rate: 1.0ml/min
Detection: UV,280nm

COMPOUNDS
1. Dextromethorphan hydrobromide imp. B
2. Dextromethorphan hydrobromide imp. C
3. Dextromethorphan hydrobromide API
4. Dextromethorphan hydrobromide imp. A

Application: paracetamol impurities 4.6mm x 250mm, 100Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 375 ml of 17.9 g/L Na2HPO4
+375 ml of 7.8 g/L
NaH2PO4 + 250 ml MeOH containing 4.6 g/L of
Tetrabutylammonium hydroxide
Flow Rate: 1.0ml/min
Detection: UV,280nm

COMPOUNDS
1. Paracetamol imp. K
2. Paracetamol API
3. Paracetamol imp. F

PuritasTM ods 120Å C-18

Application: Local Anaesthetics 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A. 50mm NaH2PO4 (pH-2.5):
ACN (95:5)
B. 50mm NaH2PO4 (pH-2.5):
ACN (47:53)
Gradient: 0-100% B in 30 min,
100% B 30-45 min
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Xylocaine
2. Benzocaine
3. Cinchocaine
4. Butamben

PuritasTM 120Å C-8

Application: Omeprazole Impurities 4.6mm x 150mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN:NaH2PO4,
pH-7.6 (27:73)
Flow Rate: 1.0ml/min
Detection: UV, 280nm

COMPOUNDS
1. Omeprazole imp. E
2. Omeprazole imp. D
3. Omeprazole API

PuritasPuritasTM 120Å C-4

Application: Local Anaesthetics 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A. 50mm NaH2PO4 (pH-2.5):
ACN (95:5)
B. 50mm NaH2PO4 (pH-2.5):
ACN (47:53)
Gradient: 0-100% B in 30 min,
100% B 30-45 min
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Xylocaine
2. Benzocaine
3. Cinchocaine
4. Butamben

PuritasTM 120Å Cn

Application: steroids 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: H2O:ACN (40:60)
Flow Rate: 0.6 ml/min
Detection: UV, 254nm

COMPOUNDS
1. Hydrocortisone
2. Estradiol
3. Levenorgesterol

Application: Diacerein Impurities 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: 0.1%
PHOSPHORIC ACID:ACN (80:20)
Flow Rate: 0.8 ml/min
Detection: UV,285nm

COMPOUNDS
1. Diacerein imp.D
2. Diacerein imp.E

PuritasTM phenyl 120Å ph

Application: Nucleic Acids 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A. 20mm Phosphate
buffer (pH-7.0)
B. 20mm Phosphate
buffer (pH-7.0): MeOH (90:10)
Gradient: 0-100% B in 20 min
Flow Rate: 1.0 ml/min
Detection: UV, 260nm

COMPOUNDS
1. Uracil
2. Guanine
3. Thymidine

PuritasTM aps

Application: Substituted Benzenes 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
Hexane: CH2Cl2 (97:3)
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Butylbenzene
2. Methylbenzoate
3. Nitrobenzene

Application: Substituted Benzenes 4.6mm x 250mm, 120Å, 5µ

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase: ACN:20mM KH2PO4,
pH-7.8 (80:20)
Flow Rate: 1.0ml/min
Detection: UV, 270nm

COMPOUNDS
1. Caffeine
2. Thymine
3. Uracil

PuritasTM DIOL

Application: Substituted Benzenes 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
Hexane: CH2Cl2 (97:3)
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Butylbenzene
2. Methylbenzoate
3. Nitrobenzene

PuritasTM SIL

Application: Substituted Benzenes 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
Hexane: IPA (100:1)
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Toluene
2. Nitrobenzene
3. m-Dinitrobenzene

Application: phenolic compounds 4.6mm x 250mm, 120Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
Hexane: EtOH (80:20)
Flow Rate: 1.0ml/min
Detection: UV, 254nm

COMPOUNDS
1. Phenol
2. Catechol
3. Resorcinol

PuritasTMbio 300Å C-18

application: lysozyme and bsa 4.6mm x 250mm, 300Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A. 0.05%TFA in H2O:ACN (80:20)
B. 0.05%TFA in H2O:ACN (40:60)
Gradient: 0-100% B in 20 min
Detection: UV, 220nm

COMPOUNDS
1. Lysozyme
2. Albumin (Bovine)

PuritasTM bio 300Å C-8

application: lysozyme and bsa 4.6mm x 250mm, 300Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A. 0.05%TFA in H2O:ACN (80:20)
B. 0.05%TFA in H2O:ACN (40:60)
Gradient: 0-100% B in 20 min
Detection: UV, 220nm

COMPOUNDS
1. Lysozyme
2. Albumin (Bovine)

PuritasTM bio 300Å C-4

application: lysozyme and bsa 4.6mm x 250mm, 300Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A. 0.05%TFA in H2O:ACN (80:20)
B. 0.05%TFA in H2O:ACN (40:60)
Gradient: 0-100% B in 20 min
Detection: UV, 220nm

COMPOUNDS
1. Lysozyme
2. Albumin (Bovine)

PuritasTM bio eximius 300Å C-18

Application: Human Insulin 4.6mm x 250mm, 300Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A: 0.1% TFA in H2O:ACN (80:20)
B: 0.1% TFA in H2O:ACN (10:90)
Gradient: 0-60% B in 15 min
Flow Rate: 1ml/min
Injection Volume: 20µl
Detection: 280nm

COMPOUNDS
1. Human Insulin

PuritasTM bio eximius 300Å C-8

Application: Human Insulin 4.6mm x 250mm, 300Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A: 0.1% TFA in H2O:ACN (80:20)
B: 0.1% TFA in H2O:ACN (10:90)
Gradient: 0-60% B in 15 min
Flow Rate: 1ml/min
Injection Volume: 20µl
Detection: 280nm

COMPOUNDS
1. Human Insulin

PuritasTM bio eximius 300Å C-4

Application: Human Insulin 4.6mm x 250mm, 300Å, 5µm

chromatograms

Chromachemie Proprietary information

CONDITIONS
Mobile phase:
A: 0.1% TFA in H2O:ACN (80:20)
B: 0.1% TFA in H2O:ACN (10:90)
Gradient: 0-60% B in 15 min
Flow Rate: 1ml/min
Injection Volume: 20µl
Detection: 280nm

COMPOUNDS
1. Human Insulin

PuritasTM prep

PURITASTMods Pn18-05-100-250 4.6mm x 250mm

chromatograms

Chromachemie Proprietary information

PURITASTM prep pp18-10-100-5008 30mm x 500mm

chromatograms

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PURITASTM prep pp18-10-100-250a 21.2mm x 250mm

chromatograms

Chromachemie Proprietary information

PURITASTM prep pp18-10-100-250c 50mm x 250mm

chromatograms

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We invite you to explore the latest innovations in Chromachemie & our latest HPLC/UPLC products and consumables, at the following conferences and exhibitions.

news and events

2 - 4 Dec. 2014

CPhI and P-MEC India

Bombay Convention and Exhibition Centre,
Mumbai, India

7 - 9 Dec. 2014

6th Annual Meet of Proteomics Society of India 2014
“Proteomics from Discovery to Function”

IIT, Mumbai, India

66th Indian Pharmaceutical Congress 2014

Dates and Venue to be announced