HPLC and UPLC Columns

Puritas Eximius C-18

Application: Aflatoxin ANALYSIS 4.6mm x 150mm, 100Å, 5µm

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CONDITIONS
Column: 4.6mm X 150mm,
C18, 5µm
Mobile phase: H2O: MeOH:
ACN (60:30:15)
Flow Rate: 1.0ml/min
Detection: FLD, Ex=365, Em=464

COMPOUNDS
1. Aflatoxin B1
2. Aflatoxin B2
3. Aflatoxin G1
4. Aflatoxin G2


APPLICATION: MALEIC AND FUMARIC ACIDS 4.6mm x 150mm, 100Å, 5µm

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CONDITIONS
Mobile phase: 50mM KH2PO4 (pH 7.0)
Flow Rate: 1.0ml/min
Detection: UV,210nm

COMPOUNDS
1. Fumaric acid
2. Maleic acid


APPLICATION: NITROANILINES 4.6mm x 150mm, 100Å, 5µm

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CONDITIONS
Mobile phase: ACN:50mM KH2PO4, pH 7.0 (50:50)
Flow Rate: 1.0ml/min
Detection: UV,254nm

COMPOUNDS
1. p-Nitroaniline
2. m-Nitroaniline
3. o-Nitroaniline


APPLICATION: ORGANIC ACIDS 4.6mm x 150mm, 100Å, 5µm

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CONDITIONS
Mobile phase: MeOH:50mM KH2PO4 , pH 5.7 (30:70)
Flow Rate: 1.0ml/min
Detection: UV,220nm

COMPOUNDS
1. L-Ascorbic acid
2. Maleic acid
3. Acetyl salicylic acid
4. Benzoic acid
5. Salicylic acid


APPLICATION: FOOD ADDITIVES 4.6mm x 250mm, 100Å, 5µm

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CONDITIONS
Mobile phase: ACN:50mM KH2PO4, pH 4.4 (40:60)
Flow Rate: 1.0ml/min
Detection: UV,230nm

COMPOUNDS
1. Phthalic acid
2. P-Hydroxy benzoic acid
3. Benzoic acid
4. Sorbic acid
5. Methyl paraben
6. Ethyl paraben
7. Propyl paraben


APPLICATION: CHLORPHENAMINE MALEATE IMPURITIES 4.6mm x 250mm, 100Å, 10µm

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CONDITIONS
Mobile phase: ACN/NH4H2PO4, pH 3.0 (20:80)
Flow Rate: 1.2ml/min
Detection: UV,225nm

COMPOUNDS
1. Maleic acid
2. Chlorphenamine maleate imp. B
3. Chlorphenamine maleate imp. C
4. Chlorphenamine maleate API
5. Chlorphenamine maleate imp. D


APPLICATION: IBUPROFEN IMPURITIES 4.6mm x 150mm, 100Å, 5µm

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CONDITIONS
Mobile phase:
A: 0.5 ml H3PO4+340 ml ACN + 660 ml H2O
B: ACN
Gradient: 0-25 min 100% A,
25-55 min 0-85% B,
55-70 min 85% B
Flow Rate: 2 ml/min
Detection: UV, 214 nm

COMPOUNDS
1. Ibuprofen imp. A
2. Ibuprofen imp. B
3. Ibuprofen API


APPLICATION: IBUPROFEN IMPURITIES 4.6mm x 150mm, 100Å, 5µm

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CONDITIONS
Mobile phase:
A: 0.5 ml H3PO4+340 ml ACN + 660 ml H2O
B: ACN
Gradient: 0-25 min 100% A,
25-55 min 0-85% B,
55-70 min 85% B
Flow Rate: 2 ml/min
Detection: UV, 214 nm

COMPOUNDS
1. Ibuprofen imp. A
2. Ibuprofen imp. B
3. Ibuprofen API


APPLICATION: DEXTROMETHORPHAN HYDROBROMIDE IMPURITIES 4.6mm x 250mm, 100Å, 5µm

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CONDITIONS
Mobile phase: 3.11 g of docusate sodium in
400 ml H2O+ 600ml ACN + 0.56 g
NH4NO3 (pH-2.0)
Flow Rate: 1.0ml/min
Detection: UV,280nm

COMPOUNDS
1. Dextromethorphan hydrobromide imp. B
2. Dextromethorphan hydrobromide imp. C
3. Dextromethorphan hydrobromide API
4. Dextromethorphan hydrobromide imp.


APPLICATION: PARACETAMOL IMPURITIES 4.6mm x 250mm, 100Å, 5µm

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CONDITIONS
Mobile phase: 375 ml of 17.9 g/L Na2HPO4
+375 ml of 7.8 g/L
NaH2PO4 + 250 ml MeOH containing 4.6 g/L of
Tetrabutylammonium hydroxide
Flow Rate: 1.0ml/min
Detection: UV,280nm

COMPOUNDS
1. Paracetamol imp. K
2. Paracetamol API
3. Paracetamol imp. F